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mbp orf24 ntd wt  (Addgene inc)


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    Structured Review

    Addgene inc mbp orf24 ntd wt
    Mbp Orf24 Ntd Wt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mbp+orf24+ntd+wt/pMAL-c2X-ORF24-NTD-3L_A+(Plasmid+%23138466)/pm32886723-178-5-7
    Average 91 stars, based on 1 article reviews
    mbp orf24 ntd wt - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Expressing:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Polymerase Chain Reaction:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Amplification:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Introduce:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Clone Assay:

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1-201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) ( ) with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [19] with primers to introduce SacI and PLOS Pathogens | https://doi.org/10.1371/journal.ppat.1008843 September 4, 2020 12 / 22 BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..

    Article Title: The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription
    Article Snippet: To generate the plasmid for GST-ORF24-NTD expression (Addgene #138464), ORF24-NTD (residues 1–201) was PCR amplified from pcDNA4/TO-ORF24-3xFLAG with primers to introduce BamHI and NotI sites and cloned into pGEX4T1 using T4 DNA ligase. pGEX4T1 encodes an N-terminal GST tag followed by a thrombin cleavage site. .. To generate the plasmid for MBP-ORF24-NTD WT (Addgene #138465) and 3L_A (Addgene #138466) expression, ORF24-NTD was PCR amplified from pcDNA4/TO-ORF24-3xFLAG WT (Addgene #138423) or 3L_A (Addgene #138425) [ ] with primers to introduce SacI and BamHI sites and cloned into pMAL-c2X using T4 DNA ligase. pMAL-c2X encodes an N-terminal MBP tag and the plasmids were cloned to express ORF24-NTD with a noncleavable MBP tag. ..



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    Addgene inc mbp orf24 ntd wt
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    https://www.bioz.com/product/mbp+orf24+ntd+wt/pMAL-c2X-ORF24-NTD-3L_A+(Plasmid+%23138466)/pm32886723-178-5-7
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